Optimization of Pulsed-Field Gel Electrophoresis for Legionella pneumophila Subtyping
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چکیده
منابع مشابه
Subtyping of Salmonella enterica isolated from humans and food animals using Pulsed-Field Gel Electrophoresis
Salmonella infections are the second leading cause of zoonotic bacterial foodborne illness. Main source of infection in human is contaminated food products. The aim of this study was sub typing isolates of Salmonella entericaobtained during our previous study byPulsed Field Gel Electrophoresis (PFGE) technique. All 46 Salmonella isolates were serotyped and then subjected to PFGE. Total isolates...
متن کاملDiscriminatory genomic fingerprinting of Legionella pneumophila by pulsed-field electrophoresis.
Eight strains of Legionella pneumophila were used to optimize cleavage of DNA with BssHII, SalI, or SpeI and separation by pulsed-field electrophoresis. Isolates from a community outbreak involving a contaminated hot tub were genomically identical. Cleavage patterns were distinctly different for unrelated environmental and nosocomial strains from a single hospital.
متن کاملAnalysis of Legionella pneumophila serogroup 1 isolates by pulsed-field gel electrophoresis.
At a hospital in Halifax, Nova Scotia, Canada, three strains of Legionella pneumophila were detectable based on plasmid content, while the isolates collected at another hospital in Halifax had no plasmids. Genomic DNA was digested with BssHII, SalI, and SpeI and subjected to pulsed-field gel electrophoresis (PFGE). We found no relationship between plasmid profile and PFGE pattern.
متن کاملSubtyping Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis.
We subtyped Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis (PFGE). This was done with 22 Brazilian Y. pestis strains: 17 from an outbreak and 5 from endemic routine surveillance. The strains were divided into 2 groups (I and II), 8 subgroups (A-H) and 19 PFGE profiles or pulsotypes. PFGE did not separate outbreak from non-outbreak strains, as identical pulsotype patterns ...
متن کاملCampylobacter Epidemiology – Insights from Subtyping by Pulsed-Field Gel Electrophoresis
Höök, H. 2005. Campylobacter epidemiology – insights from subtyping by pulsed-field gel electrophoresis. Doctoral thesis. ISSN 1652-6880, ISBN 91-576-6988-0 Campylobacter jejuni and Campylobacter coli are frequent worldwide causes of food-borne gastroenteritis. Poultry is regarded as the most important infection source. In temperate countries, a marked seasonal variation of human campylobacteri...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2010
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01455-09